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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Effect of different water regime on microorganisms of nitrogen cycle in permafrost
 TURKOVÁ, Sára
The gene abundances and community structures of nitrogen-fixing and denitrifying bacteria in permafrost-affected soils with different moisture level were analysed. Additionally, DNA- and RNA-based analysis was used to evaluate the variations in gene copy numbers and community composition between the total and active bacteria. The gene abundances of nitrogen-fixing and denitrifying bacteria were quantified by qPCR of the nifH and nosZ genes, and the gene copy numbers were compared between the soils with different moisture. Furthermore, the bacterial community structure variation was evaluated by analysis of the taxonomic classification obtained from sequencing data.
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Quantification of specific microbial functional guilds in Arctic permafrost soil
 WALTER, Deborah
The quantity of specific bacterial and archaeal groups were determined in permafrost soils. The selected groups were quantified by quantitative polymerase chain reaction (qPCR) of specific genes: total archaea (16SrRNA gene), methanogenic archaea (mcrA gene), nitrogen fixators (nifH gene) and denitrifiers (nosZ gene). The quantities of these groups were compared between different soil horizons and furthermore correlations between the quantity of gene copies and basic soil parameters were computed.
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